Isogenic Strains of PHYCOMYCES BLAKESLEEANUS Suitable for Genetic Analysis
AUTOR(ES)
Alvarez, M. I.
RESUMO
The progeny of crosses between wild-type strains of Phycomyces usually do not exhibit all of the expected genotypes from meiosis. By backcrossing, we have isolated a new (+) mating-type strain, A56, which is nearly isogenic with the (-) wild-type NRRL1555 commonly used in Phycomyces research. Tetrad analysis of the backcrosses shows that meiosis becomes more regular as the parental (+) and (-) strains become more isogenic. In our two-factor crosses with unlinked markers, the regularity of meiosis is measured as the percent of reciprocal ditypes plus tetratypes in the progeny. We have shown that this percentage increases from about 15% for crosses between nonisogenic parents to 90% in the eighth backcross. The results indicate that routine, reliable recombination analyses are possible in P. blakesleeanus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1202231Documentos Relacionados
- Stereospecific analysis of major glycerolipids of Phycomyces blakesleeanus sporangiophores and mycelium.
- Isolation and characterization of Phycomyces blakesleeanus ferritin.
- Mutations affecting sexual development in Phycomyces blakesleeanus.
- Effect of glucose on isocitrate lyase in Phycomyces blakesleeanus.
- Nucleotide sequence of the Phycomyces blakesleeanus leu1 gene.
