Isolation and characterization of a lambdapolA transducing phage.
AUTOR(ES)
Kelley, W S
RESUMO
A plaque-forming lambdapolA phage was isolated from a population of transducing phage made in vitro from Escherichia coli DNA and a phage vector digested with restriction endonuclease HindIII. Amber mutations, in genes whose products are necessary for late protein synthesis (Q) and cell lysis (S), were crossed into the lambdapolA phage. Infection of either polA+ or polA- bacteria with this phage, under conditions permitting DNA replication but preventing phage production and lysis, elevated the levels of DNA polymerase I to between 75- and 100-fold that detected in a wild-type strain. The kinetics of enzyme production suggest that the polA gene is transcribed from its own promoter rather than from any of the well-characterized phage promoters. The fragment of E. coli DNA within the lambdapolA phage comprises approximately 5000 base pairs, sufficient to accommodate the polA gene and one, or two, coding sequences for smaller proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=431839Documentos Relacionados
- Isolation and sequencing of gene fusions carried by lambda placMu specialized transducing phage.
- Chemotaxis in Escherichia coli: construction and properties of lambda tsr transducing phage.
- Isolation and characterization of a generalized transducing phage for Xanthomonas campestris pv. campestris.
- Fis is required for illegitimate recombination during formation of lambda bio transducing phage.
- Isolation and Characterization of a Generalized Transducing Phage for Pseudomonas aeruginosa Strains PAO1 and PA14
