Isolation and characterization of Escherichia coli mutants blocked in production of membrane-derived oligosaccharides.
AUTOR(ES)
Weissborn, A C
RESUMO
We report a new procedure for the facile selection of mutants of Escherichia coli that are blocked in the production of membrane-derived oligosaccharides. Four phenotypic classes were identified, including two with a novel array of characteristics. The mutations mapped to two genetic loci. Mutations in the mdoA region near 23 min are in two distinct genes, only one of which is needed for the membrane-localized glucosyltransferase that catalyzes the synthesis of the beta-1,2-glucan backbone of membrane-derived oligosaccharides. Another set of mutations mapped near 27 min closely linked to osmZ; these appear to be in the galU gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=206289Documentos Relacionados
- Osmotic regulation of biosynthesis of membrane-derived oligosaccharides in Escherichia coli.
- Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.
- Transfer of phosphoethanolamine residues from phosphatidylethanolamine to the membrane-derived oligosaccharides of Escherichia coli.
- Biosynthesis of membrane-derived oligosaccharides: characterization of mdoB mutants defective in phosphoglycerol transferase I activity.
- Biosynthesis of membrane-derived oligosaccharides: a periplasmic phosphoglyceroltransferase.
