Isolation and characterization of fecal bacteria capable of 16 alpha-dehydroxylating corticoids.
AUTOR(ES)
Bokkenheuser, V D
RESUMO
For more than a decade it has been known that the fecal flora of humans and rats includes organisms capable of 16 alpha-dehydroxylating corticoids, but their identity has remained unknown. To isolate these organisms, Mueller-Hinton agar plates were seeded with fresh feces from Proteus-free rats and incubated anaerobically. On an average, 1 of every 35 colonies consisted of organisms synthesizing 16 alpha-dehydroxylase. Isolation of the individual colonies yielded two obligate anerobes, strains 144 and 146, which elaborated the enzyme. The steroid transformation could be attained by the microbial culture alone in prereduced media or in aerobic media in the presence of Escherichia coli. Although both strains were phenotypically similar to Eubacterium lentum, they differed between themselves in their enzymatic equipment.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=291664Documentos Relacionados
- Isolation and characterization of human fecal bacteria capable of 21-dehydroxylating corticoids.
- Isolation and characterization of thirteen intestinal microorganisms capable of 7 alpha-dehydroxylating bile acids.
- Assessment of fecal bacteria with bile acid 7 alpha-dehydroxylating activity for the presence of bai-like genes.
- Isolation and Characterization of Two Enzymes Capable of Hydrolyzing Fructose-1,6-Bisphosphatase from the Lichen Peltigera rufescens1
- Isolation and identification of fecal bacteria from adult swine.