Isolation and Properties of Enterococcus hirae Mutants Defective in the Potassium/Proton Antiport System

AUTOR(ES)
FONTE

American Society for Microbiology

RESUMO

A K+/H+ antiporter regulates cytoplasmic pH in Enterococcus hirae growing at alkaline pH. Mutants defective in this antiport activity were alkaline pH sensitive. One mutant, Pop1, lacked both K+/methylamine exchange at pH 9.5 and concomitant acidification of cytoplasmic pH. Pop1 grew well at pHs below 8 but did not at pHs above 9, conditions under which cytoplasmic pH was not fully acidified.

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