Isolation, mapping, and examination of effects of TnA insertions in ColE1 plasmids.

AUTOR(ES)

Inselburg, J

RESUMO

Twelve TnA insertions of ColE1 deoxyribonucleic acid have been isolated and mapped by electron microscopic studies of heteroduplex molecules. Insertions only blocking the production of active colicin clustered in one region of the map, whereas insertions only blocking the expression of deoxyribonucleic acid nicking activity associated with the plasmid "relaxation complex" clustered in another region of the map. The location of one insertion that blocks both the expression of colicin immunity and the production of active colicin suggests that the expression of both characteristics are coordinately controlled.

Documentos Relacionados

• Isolation and characterization of replication-deficient mutants of ColE1 plasmids.
• Homology between Escherichia coli plasmids ColE1 and p15A.
• Strand-Specific Supercoiled DNA-Protein Relaxation Complexes: Comparison of the Complexes of Bacterial Plasmids ColE1 and ColE2
• Alternative forms of lethality in mitomycin C-induced bacteria carrying ColE1 plasmids
• Isolation and genetic analysis of deletion mutants of colicin E1 plasmids carrying a TnA insertion.