Isolation of a New RNA Polymerase-Binding Protein from Sporulating Bacillus subtilis

AUTOR(ES)

Greenleaf, Arno L.

RESUMO

RNA polymerase was precipitated from extracts of radioactively labeled vegetative and sporulating Bacillus subtilis with antiserum prepared against vegetative core polymerase. The precipitates were solubilized and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Antiserum added to an extract of vegetative B. subtilis precipitated only the known subunits of core RNA polymerase, but antiserum added to an extract of sporulating cells precipitated a new polypeptide of 70,000 daltons in addition to the subunits of core enzyme. The 70,000-dalton polypeptide precipitated from an extract of a mixture of vegetative and sporulating B. subtilis, separately labeled with two different radioisotopes, contained only the radioisotope characteristic of the sporulating cells.

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