Isolation of DNA-Membrane Complex in Bacillus subtilis
AUTOR(ES)
Sueoka, Noboru
RESUMO
A DNA-membrane complex of Bacillus subtilis was separated from the bulk of the membrane by CsCl-sucrose double gradient centrifugation. The complex has a CsCl-sucrose buoyant density of 1.35-1.45 g/cm3. Gel electrophoretic analyses show that the complex has about 5% of the whole membrane proteins and contains proteins unique to the complex as well as a small amount of membrane proteins. DNA in the complex is enriched for a genetic marker close to the origin (pur A16) and for one near the terminus (metB5). Artifactual formation of the complex during the lysate preparation was shown to be unlikely.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433982Documentos Relacionados
- Initiation of DNA replication in vitro by a DNA-membrane complex extracted from Bacillus subtilis.
- Characterization of a multienzyme complex derived from a Bacillus subtilis DNA-membrane extract that synthesizes RNA and DNA precursors.
- Chromosome-membrane association in Bacillus subtilis. IV. Further purification of DNA-membrane complex by using a combination of centrifugation and electrophoresis.
- DNA replication by a DNA-membrane complex extracted from Bacillus subtilis: site of initiation in vitro and initiation potential of subcomplexes.
- DNA-membrane association is necessary for initiation of chromosomal and plasmid replication in Bacillus subtilis.
