Isolation of Francisella tularensis from infected frozen human blood.
AUTOR(ES)
Pittman, B
RESUMO
Francisella tularensis was isolated from human blood that was frozen for 3 months before it was examined. Before he became ill, the patient operated a "bush-hog" in an area thickly populated with rabbits. His illness was undiagnosed and untreated before his death. Portions of blood and tissue homogenates from necropsy were injected intraperitoneally into mice and inoculated onto glucose-cysteine-blood agar plates. F. tularensis did not grow from the culture plates, but mice inoculated with the blood died in 48 to 72 h. Fluorescent-antibody stains of mouse liver and spleen impression smears showed clumps of cells or amorphous masses of brightly staining envelope material around the cells. Tissue impressions of liver, spleen, and heart blood inoculated onto glucose-cysteine-blood agar yielded pure cultures of F. tularensis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=274667Documentos Relacionados
- Isolation of Francisella tularensis from blood.
- Isolation of a novel endogenous opiate analgesic from human blood.
- Modification of standard proteinase K/phenol method for DNA isolation to improve yield and purity from frozen blood.
- Properties of Aldolase from Francisella tularensis
- Detection of Francisella tularensis in blood by polymerase chain reaction.
