Isolation of Haemophilus influenzae genes that suppress Escherichia coli polA mutations.

AUTOR(ES)

Williams, G L

RESUMO

Haemophilus influenzae was found to produce a DNA polymerase that was similar to polymerase I of Escherichia coli. E. coli polA mutants were used as backgrounds for the selection of H. influenzae polA suppressor genes. Six different H. influenzae fragments were isolated that could suppress E. coli polA mutations. None of the suppressors appeared to encode the H. influenzae equivalent of the E. coli polA gene. One type of clone, represented by pGW41, caused a polymerase I activity to appear in a suppressed polA1 mutant. Plasmids from the pGW41 class contained two genes (pol-2 and pol-3) that were both required for polA suppression. Mutated nonsuppressing derivatives of the pGW41 class were used to create H. influenzae mutants that were deficient in polymerase I.

Documentos Relacionados

• Identification of two genes immediately downstream from the polA gene of Escherichia coli.
• Thymineless Death in polA+ and polA− Strains of Escherichia coli
• Streptococcus pneumoniae polA gene is expressed in Escherichia coli and can functionally substitute for the E. coli polA gene.
• Suppression of ColE1 high-copy-number mutants by mutations in the polA gene of Escherichia coli.
• Ultraviolet-Sensitive Mutator mutU4 of Escherichia coli Inviable with polA