Isolation of maxicircle component of kinetoplast DNA from hemoflagellate protozoa.
AUTOR(ES)
Simpson, L
RESUMO
Maxicircle DNA has been isolated from the kinetoplast DNA of Leishmania tarentolae culture forms by bouyant separation in CsCl in the presence of the A + T-binding dye Hoechst 33258, after liberation from the kinetoplast DNA network by cleavage with the single-hit restriction endonuclease EcoRI. The purified linearized maxicircle DNA has a density in CsCl of 1.681 g/cm3 (79% A + T) and a molecular weight of approximately 18-20 x 10(6). The maxicircle molecule exhibited intramolecular base composition heterogeneity ranging from 85% A + T to 74% A + T.
