Isolation of nit-4, the minor nitrogen regulatory gene which mediates nitrate induction in Neurospora crassa.
AUTOR(ES)
Fu, Y H
RESUMO
Expression of nitrate reductase in Neurospora crassa requires the positive action of nit-4, a pathway-specific regulatory gene, which mediates nitrate induction. We report the molecular cloning of the nit-4 gene and present results which suggest that the nit-4 gene is constitutively expressed to yield a low-abundance 2.2-kilobase transcript. These results indicate that the nit-2 major control gene and the nit-4 pathway-specific control gene independently regulate the expression of the nitrate assimilatory structural genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=210163Documentos Relacionados
- Characterization of nit-2, the major nitrogen regulatory gene of Neurospora crassa.
- Binding affinity and functional significance of NIT2 and NIT4 binding sites in the promoter of the highly regulated nit-3 gene, which encodes nitrate reductase in Neurospora crassa.
- Metabolic control and autogenous regulation of nit-3, the nitrate reductase structural gene of Neurospora crassa.
- Nitrogen metabolite repression of nitrate reductase in Neurospora crassa.
- nit-4, a pathway-specific regulatory gene of Neurospora crassa, encodes a protein with a putative binuclear zinc DNA-binding domain.
