Isolation of plasmid deoxyribonucleic acid from Pseudomonas putida.
AUTOR(ES)
Palchaudhuri, S
RESUMO
Conditions suitable for reproducible recovery of covalently closed circular deoxyribonucleic acid from strains of Pseudomonas putida containing degradative plasmids (CAM, SAL, OCT, etc.) have been defined. These degradative plasmids could not be isolated by the usual procedure, whereas RP1, an R factor of the P group, present in the isogenic strain of P. putida, was isolated equally well by either the usual procedure or the modified procedure. Characterization by electron microscopy of RP1 deoxyribonucleic acid confirmed the molecular weight (about 40 X 10(6)) previously determined by sucrose gradient centrifugation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=233297Documentos Relacionados
- Isolation of a third lipoamide dehydrogenase from Pseudomonas putida.
- Isolation of a specific lipoamide dehydrogenase for a branched-chain keto acid dehydrogenase from Pseudomonas putida.
- Chromosomal location of TOL plasmid DNA in Pseudomonas putida.
- Dissociation of the NIC plasmid aggregate in Pseudomonas putida.
- Purification of a branched-chain keto acid dehydrogenase from Pseudomonas putida.