Isolation of the Ends of La Crosse Virus Small RNA as a Double-Stranded Structure

AUTOR(ES)

Patterson, Jean L.

RESUMO

The genome of La Crosse virus, a member of the Bunyaviridae, is made up of three molecules. Circular nucleocapsid structures, in three size classes, have been isolated from La Crosse virus (Obijeski et al. J. Virol. 20:664-675, 1976). Recently, Obijeski et al. (Nucleic Acids Res. 8:2431-2438) have found that the 5′ and 3′ ends of each segment are complementary in sequence. We determined that a 5′ and 3′ end complementary structure, predicted by the rules of Tinoco et al. (Nature [London] 230:362-367), can and will anneal under certain conditions. This structure is resistant to RNase in high-salt medium and can be isolated in a reasonably high yield.

Documentos Relacionados

• A deletion mutant of L-A double-stranded RNA replicates like M1 double-stranded RNA.
• Packaging in a yeast double-stranded RNA virus.
• The Core of Bluetongue Virus Binds Double-Stranded RNA
• Molecular cloning of double-stranded RNA virus genomes.
• The coat protein of the yeast double-stranded RNA virus L-A attaches covalently to the cap structure of eukaryotic mRNA.