Isolation of Tissue-Specific cDNAs from Tomato Pistils.
AUTOR(ES)
Gasser, C. S.
RESUMO
We have used a differential plaque hybridization screening procedure to isolate cDNA clones for genes that show elevated or exclusive expression in tomato pistils. Clones that showed maximal expression in immature pistils (premeiotic to early meiosis) and mature pistils (at anthesis) were isolated. Of nine clones that were characterized, four were found also to express at some stage of anther development. In situ hybridization experiments showed that expression of the genes we have identified is very tightly regulated both spatially and temporally within the pistil. One gene was identified that is expressed in the pistil only in the transmitting tissue of the style. A second gene was found to express exclusively in two to three cell layers of the ovules for a period of less than eight days.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=159733Documentos Relacionados
- Ovule development: identification of stage-specific and tissue-specific cDNAs.
- Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart.
- Tissue-specific overexpression of lipoprotein lipase causes tissue-specific insulin resistance
- A novel method for the isolation of tissue-specific genes.
- Synthesis of sperm and late histone cDNAs of the sea urchin with a primer complementary to the conserved 3' terminal palindrome: evidence for tissue-specific and more general histone gene variants.
