Isolation of TOL and RP4 recombinants by integrative suppression.

AUTOR(ES)

Nakazawa, T

RESUMO

We obtained genetic and molecular evidence of non-thermosensitive recombinants of RP4 (Kmr Tcr Cbr/Apr) and the thermosensitive TOL plasmid. As first isolated in Pseudomonas aeruginosa PAO, the recombinant plasmid pTN1 specified noninducible synthesis of TOL enzymes and was transmissible to Escherichia coli on selection for the transfer of kanamycin resistance. The phenotypic expression of TOL genes of pTN1 in E. coli was low and also noninducible. A spontaneous segregant, pTN2, appearing from pTN1, conferred inducible synthesis of TOL enzymes. These plasmids carry all of the TOL determinants as evidenced by the ability of Pseudomonas putida carrying recombinant plasmids to grow on toluene, xylene, and m-toluate. In E. coli the expression of TOL genes with normal regulation (pTN2) appears to be extremely low without induction, and the induced expression is comparable to that with defective regulation (pTN1). The measurement of the molecular weight of pTN2 by electron microscopy gave a value of about 74 X 10(6).

Documentos Relacionados

• Physical and functional mapping of RP4-TOL plasmid recombinants: analysis of insertion and deletion mutants.
• Activity of Plasmid Replicons in CAULOBACTER CRESCENTUS : Rp4 and Cole1
• Formation of Escherichia coli Hfr strains by integrative suppression with the P group plasmid RP1.
• Rp4 Promotion of Transfer of a Large Agrobacterium Plasmid Which Confers Virulence
• The mating pair formation system of plasmid RP4 defined by RSF1010 mobilization and donor-specific phage propagation.