Isolation of Uridine 5′-Pyrophosphate Glucuronic Acid Pyrophosphorylase and Its Assay Using 32P-Pyrophosphate 1

AUTOR(ES)

Dickinson, David B.

RESUMO

A procedure was devised to detect and assay uridine 5′-pyrophosphate (UDP)-glucuronic acid pyrophosphorylase in plant extracts. Substrates are UDP-glucuronic acid and 32P-pyrophosphate, and the 32P-uridine 5′-triphosphate produced is selectively adsorbed to charcoal. The charcoal adsorption procedure is a modification of that used to determine 32P-adenosine 5′-triphosphate produced by adenosine 5′-pyrophosphate glucose pyrophosphorylase, and the modification greatly improves the retention of uridine 5′-triphosphate.

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