Isolation of Z-DNA-containing plasmids.
AUTOR(ES)
Thomae, R
RESUMO
A purified, monoclonal antibody, specific for the left-handed Z-form of poly(dG-dC), was coupled covalently to Sephacryl S-1000 beads. Such an antibody column provides a convenient method to isolate and purify those plasmid DNAs that contain Z-DNA from a large excess of other DNAs, RNA, etc. From a library of Escherichia coli DNA, cloned into the vector plasmid pUC-8, several recombinant plasmids were isolated, which bind to this antibody. Thus, E. coli contains sequences, which in "natural" negatively supercoiled DNA, adopt a left-handed Z-DNA-like conformation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=384295Documentos Relacionados
- Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids.
- Novel densitometric method for endonuclease analysis of Escherichia coli DNA samples containing multiple plasmids.
- Intramolecular DNA triplexes in supercoiled plasmids.
- Conformational flexibility of junctions between contiguous B- and Z-DNAs in supercoiled plasmids.
- Isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids.