Karyotyping of Single Human Chromosomes from Dry Mass Determined by Electron Microscopy

AUTOR(ES)

Bahr, Gunter F.

RESUMO

Human chromosomes from cultures of peripheral lymphocytes were prepared for electron microscopy by surface spreading and critical-point drying. Total and partial determinations of chromosomal dry mass were made from contrast in electron microscopic negatives. The distribution of dry weights was analyzed with respect to the Denver-Chicago classifications. There is considerable variability of mass within each group, but no statistically significant differences between four donors. A single chromosome can be correctly classified from its total mass and the ratio of long- to short-arm mass.

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