Kinetics of Genetic Recombination in ESCHERICHIA COLI K-12: Competition between Genetic Integration and Degradation
AUTOR(ES)
Ou, Jonathan T.
RESUMO
The effect of shifting zygotes from a rich medium into a poor medium (shift-down) on the frequency of recombination has been examined; our results suggest that protein syntheses other than those required for growth may be involved. Experiments with chloramphenicol support this notion, and further suggest that there is a competition between genetic integration and degradation in the recipient cells. By inhibiting protein synthesis one can shift the competition in favor of either integration or segregation, depending on the physiological state of the zygotes. By calculating the time required for segregation as one generation, we conclude that the recombination chromosome is homozygous at the time of integration.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1213031Documentos Relacionados
- General recombination in Escherichia coli K-12: in vivo role of RecBC enzyme.
- Competition between congenic Escherichia coli K-12 strains in vivo.
- L-serine degradation in Escherichia coli K-12: cloning and sequencing of the sdaA gene.
- Threonine Locus of Escherichia coli K-12: Genetic Structure and Evidence for an Operon
- L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants.
