Kinetics of T cell-activation molecules in response to Mycobacterium tuberculosis antigens
AUTOR(ES)
Antas, Paulo RZ, Oliveira, Eliane B, Milagres, Alexandre S, Franken, Kees C, Ottenhoff, Tom HM, Klatser, Paul, Sarno, Euzenir N, Sampaio, Elizabeth P
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2002-12
RESUMO
The phenotypic features acquired subsequent to antigen-specific stimulation in vitro were evaluated by means of the kinetic expressions of CD69 and CD25 activation molecules on T lymphocytes and assayed by flow cytometry in response to PPD, Ag85B, and ferritin in PPD-positive healthy control individuals. In response to PHA, CD69 staining on both CD4+ and CD8+ T cells became initially marked after 4 h, peaked at 24 h, and quickly decreased after 120 h. For CD25, a latter expression was detected around 8 h, having increased after 96 h. As expected, the response rate to the mycobacterial antigens was much lower than that to the mitogen. Positive staining was high after 96 h for CD25 and after 24 h for CD69. CD69 expression was significantly enhanced (p < 0.05) on CD8+ as compared to CD4+ T cells. High levels were also found between 96-120 h. Regarding Ag85B, CD25+ cells were mostly CD4+ instead of CD8+ T cells. Moreover, in response to ferritin, a lower CD25 expression was noted. The present data will allow further characterization of the immune response to new mycobacterial-specific antigens and their evaluation for possible inclusion in developing new diagnostic techniques for tuberculosis as well in a new vaccine to prevent the disease.
Documentos Relacionados
- T-cell proliferative response to antigens secreted by Mycobacterium tuberculosis.
- Human immune response to Mycobacterium tuberculosis antigens.
- T-Cell Responses to CD1-Presented Lipid Antigens in Humans with Mycobacterium tuberculosis Infection
- Kinetics and Immunoglobulin Nature of the Antibody Response to Mycobacterium tuberculosis
- Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals