Lac Operator Analogues: Bromodeoxyuridine Substitution in the lac Operator Affects the Rate of Dissociation of the lac Repressor
AUTOR(ES)
Lin, Syr-Yaung
RESUMO
As measured by a decreased rate of dissociation, lac repressor binds 10-times tighter to 5-bromodeoxyuridine-substituted lac operator than it does to normal lac operator. This result is obtained both in the absence and in the presence of isopropylthiogalactoside, an inducing ligand. These data are significant with regard to the mechanism of sequence-specific protein-DNA interaction, and also suggest a possible explanation for the effects of bromodeoxyuridine on the expression of differentiated functions in eukaryotic cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=426991Documentos Relacionados
- Photochemical Attachment of lac Repressor to Bromodeoxyuridine-Substituted lac Operator by Ultraviolet Radiation
- How lac repressor recognizes lac operator.
- lac repressor-lac operator interaction: NMR observations.
- Contacts between the lac repressor and the thymines in the lac operator.
- The interaction of the recognition helix of lac repressor with lac operator.
