LaeA, a Regulator of Secondary Metabolism in Aspergillus spp.
AUTOR(ES)
Bok, Jin Woo
FONTE
American Society for Microbiology
RESUMO
Secondary metabolites, or biochemical indicators of fungal development, are of intense interest to humankind due to their pharmaceutical and/or toxic properties. We present here a novel Aspergillus nuclear protein, LaeA, as a global regulator of secondary metabolism in this genus. Deletion of laeA (ΔlaeA) blocks the expression of metabolic gene clusters, including the sterigmatocystin (carcinogen), penicillin (antibiotic), and lovastatin (antihypercholesterolemic agent) gene clusters. Conversely, overexpression of laeA triggers increased penicillin and lovastatin gene transcription and subsequent product formation. laeA expression is negatively regulated by AflR, a sterigmatocystin Zn2Cys6 transcription factor, in a unique feedback loop, as well as by two signal transduction elements, protein kinase A and RasA. Although these last two proteins also negatively regulate sporulation, ΔlaeA strains show little difference in spore production compared to the wild type, indicating that the primary role of LaeA is to regulate metabolic gene clusters.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=387652Documentos Relacionados
- A putative two-component regulatory system involved in secondary metabolism in Streptomyces spp.
- Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius.
- N-acetylcysteine inhibits germination of conidia and growth of Aspergillus spp. and Fusarium spp.
- A Circadian Oscillator in Aspergillus spp. Regulates Daily Development and Gene Expression
- Zinc-Regulated Biosynthesis of Immunodominant Antigens from Aspergillus spp.
