Lanthanide Ion-Induced Isotropic Shifts and Broadening for Nuclear Magnetic Resonance Structural Analysis of Model Membranes

AUTOR(ES)

Andrews, S. Brian

RESUMO

The effects of a series of aquated lanthanide ions on the nuclear magnetic resonance spectrum of phospholipid bilayer suspensions are reported. The ability of these ions to provide a spectral distinction between morphologically exterior resonances and their interior counterparts was confirmed. Measurements of the relative shifting and broadening capabilities of these ions are reported, and their correlation with solid-state magnetic susceptibility anisotropies is shown to implicate a dipolar (pseudocontact) mechanism as the source of this effect. The potential for the use of dipolar shifts as structural probes is discussed and an alternative method using dipolar shifting and broadening reagents simultaneously is presented.

Documentos Relacionados

• Zinc Ion-induced Domain Organization in Metallo-β-lactamases: A FLEXIBLE “ZINC ARM” FOR RAPID METAL ION TRANSFER?*
• Dissection of the ion-induced folding of the hammerhead ribozyme using 19F NMR
• Nuclear Magnetic Resonance Shift Reagents: Abnormal 13C Shifts Produced by Complexation of Lanthanide Chelates with Saturated Amines and n-Butyl Isocyanide
• Greater hydrogen ion-induced depression of tension and velocity in skinned single fibres of rat fast than slow muscles.
• Identification of an interactor of cadmium ion-induced glycine-rich protein involved in regulation of callose levels in plant vasculature