Large-Scale Production of Coenzyme F420-5,6 by Using Mycobacterium smegmatis
AUTOR(ES)
Isabelle, Dale
FONTE
American Society for Microbiology
RESUMO
Production of coenzyme F420 and its biosynthetic precursor FO was examined with a variety of aerobic actinomycetes to identify an improved source for these materials. Based on fermentation costs, safety, and ease of growth, Mycobacterium smegmatis was the best source for F420-5,6. M. smegmatis produced 1 to 3 μmol of intracellular F420 per liter of culture, which was more than the 0.85 to 1.0 μmol of F420-2 per liter usually obtained with Methanobacterium thermoautotrophicum and ∼10-fold higher than what was previously reported for the best aerobic actinomycetes. An improved chromatography system using rapidly flowing quaternary aminoethyl ion-exchange material and Florisil was used to more quickly and easily purify F420 than with previous methods.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=129890Documentos Relacionados
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