Legionella pneumophila Contains a Type II General Secretion Pathway Required for Growth in Amoebae as Well as for Secretion of the Msp Protease

AUTOR(ES)

Hales, Laura M.

FONTE

American Society for Microbiology

RESUMO

We report the identification of a set of Legionella pneumophila genes that encode products with homology to proteins of the type II general secretion pathway of gram-negative bacteria. A strain containing a deletion-substitution mutation of two of these genes was unable to secrete the Msp protease. This strain was unable to multiply within the free-living amoeba Acanthamoeba castellanii yet was able to kill HL-60-derived macrophages. Because Msp is not required for growth in amoebae, other proteins which are important for growth in amoebae are likely secreted by this pathway.

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