Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene.

AUTOR(ES)

Thorbjarnardóttir, S

RESUMO

We describe the cloning and the DNA sequence of an amber suppressor allele of the Escherichia coli leuX (supP) gene. The suppressor allele codes for a tRNA with anticodon CUA, presumably derived by a single base change from a CAA anticodon. The mature coding sequence of the leuX gene is preceded by a putative Pribnow box sequence (TATAAT) and followed by a termination signal. The sequence of the leuX-coded tRNA is compared with the sequences of the four remaining tRNALeu isoacceptors of E. coli and with two tRNALeu species from bacteriophage T4 and T5. The conserved nucleotides in these seven tRNAs recognized by E. coli leucyl-tRNA synthetase are located mainly in the aminoacyl stem and in the D-stem/loop region.

Documentos Relacionados

• The nucleotide sequence of a human serine tRNA gene.
• A novel function of RNase P from Escherichia coli: processing of a suppressor tRNA precursor.
• The nucleotide sequence of asparagine tRNA from Escherichia coli.
• Nucleotide Sequence in the Promoter Region of the Escherichia coli Tyrosine tRNA Gene*
• Nucleotide sequence of a wheat mitochondrial glutamine tRNA gene.