Leukotriene D: a slow reacting substance from rat basophilic leukemia cells.
AUTOR(ES)
Orning, L
RESUMO
A slow reacting substance produced by rat basophilic leukemia cells, treated with ionophore A23187, was characterized by spectroscopic methods, enzymatic conversions, and chemical degradations as 5-hydroxy-6-S-cysteinylglycyl 7,9,11,14-eicosatetraenoic acid (leukotriene D). gamma-Glutamyltranspeptidase [gamma-glutamyltransferase; (5-glutamyl)-peptide: amino-acid 5-glutamyltransferase, EC 2.3.2.2] converted leukotriene C to a product identical to leukotriene D. This suggests that the stereochemistry of the arachidonyl moiety of leukotrienes C and D is the same [5(S)-6(R)-7,9-trans-11,14-cis]. Leukotriene D induces a faster contraction and, on a molar basis, is more potent than leukotriene C in the isolated guinea pig ileum bioassay.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=348641Documentos Relacionados
- Precursor role of arachidonic acid in release of slow reacting substance from rat basophilic leukemia cells.
- Leukotriene C: a slow-reacting substance from murine mastocytoma cells.
- Isolation and characterization of leukotriene C4 synthetase of rat basophilic leukemia cells.
- Characterization of slow reacting substances (SRSs) of rat basophilic leukemia (RBL-1) cells: effect of cysteine on SRS profile.
- Release of a slow-reacting substance from rabbit platelets.
