Local antibody response in chickens: analysis of antibody synthesis to Newcastle disease virus by solid-phase radioimmunoassay and immunofluorescence with class-specific antibody for chicken immunoglobulins.
AUTOR(ES)
Ewert, D L
RESUMO
The antibody response to Newcastle disease virus was monitored in the sera and salivas of adult chickens immunized by two methods: (i) combined intratracheal-intranasal vaccination followed by intratracheal revaccination or (ii) intramuscular vaccination followed by intratracheal revaccination. By solid-phase radioimmunoassay, only immunoglobulin G (IgG) and IgA antibodies to Newcastle disease virus were detected in the salivas, whereas IgA and IgM antibodies were present in egg whites. The first method produced the highest antibody levels in both serum and saliva and, in addition, prevented detectable virus multiplication in the respiratory tracts upon revaccination 4 weeks later. Plasma cells of all three classes were distributed throughout the tissues lining the oral cavities. The highest densities of plasma cells were in the Harderian glands; IgG was the predominant class, whereas IgA and IgM plasma cells were present in almost equal but lower numbers. The Harderian plasma cells were the most likely source of the antibody found in saliva.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=414292Documentos Relacionados
- Characterization of murine antibody response to Salmonella typhimurium by a class-specific solid-phase radioimmunoassay.
- Solid-phase micro-radioimmunoassay to measure immunoglobulin class-specific antibody to Mycoplasma pulmonis.
- Filter paper solid-phase radioimmunoassay for human rotavirus surface immunoglobulins.
- Mumps Class-Specific Immunoglobulins in Radioimmunoassay and Conventional Serology
- Specific immunoglobulin M response to hepatitis A virus determined by solid-phase radioimmunoassay.