Localization of Adenovirus 2 Messenger RNAs to Segments of the Viral Genome Defined by Endonuclease R·R1

AUTOR(ES)
RESUMO

Adenovirus 2 mRNAs synthesized in productive infection were assigned to specific regions of the genome by hybridization to unique fragments of viral DNA. Radioactive viral RNA synthesized early or late in infection was first fractionated by polyacrylamide gel electrophoresis. Eluted RNAs were then tested for complementary hybrid formation with each of the six fragments of adenovirus 2 DNA generated by cleavage with endonuclease R·R1.

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