Localization of Replicating DNA of Simian Virus 40 in Monkey Kidney Cells
AUTOR(ES)
LeBlanc, Donald J.
RESUMO
Approximately 80-85% of the DNA synthesized by cells infected with simian virus 40 during a short pulse with [3H]thymidine is associated with host nuclear membrane components, as defined by the M-band technique. A substantial portion of this membrane-associated DNA has been shown to be viral specific by sedimentation studies and by DNA·DNA hybridization. Examination of DNA associated and unassociated with nuclear M-band by neutral sucrose gradient analysis suggested that while replicating DNA of simian virus 40 is attached to the nuclear membrane, mature component I DNA of simian virus 40 is released from the membrane upon completion of replication.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388426Documentos Relacionados
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