Localization of the site of recombination in formation of the Lepore Boston globin gene.
AUTOR(ES)
Baird, M
RESUMO
The site of crossover between the delta- and beta-globin gene sequences resulting in Lepore Boston globin gene formation has been localized at the DNA Level. Probes specific for detecting the intervening sequences (IVS) of the delta- and beta-globin genes were used to map the origin of cellular DNA fragments of a patient homozygous for hemoglobin Lepore Boston. Restriction endonuclease analysis and hybridization of this DNA to IVS specific probes show that most, if not all, of the large intervening sequences (IVS 2) in Lepore DNA are derived from the beta-globin gene IVS 2. In addition, the crossover occurs in a region of DNA in which the delta- and beta-genes have almost complete nucleotide homology, and might be expected to pair most tightly during meiosis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=370832Documentos Relacionados
- Haemoglobin LeporeBoston in a Turkish family.
- Haemoglobin Lepore Boston and elliptocytosis in a family of Indonesian-German ancestry.
- Haemoglobin Lepore Boston-Washington in Sicily: clinical, haematological, and biosynthetic studies.
- A common mutant EcoRI restriction endonuclease site in the 5' flanking portion of the human alpha-globin gene.
- Recombination and balanced chromosome polymorphism suggested by DNA sequences 5' to the human delta-globin gene.