Localized Mutagenesis for the Isolation of Temperature-Sensitive Mutants of ESCHERICHIA COLI Affected in Protein Synthesis
AUTOR(ES)
Champney, W. Scott
RESUMO
Two variations of the method of localized mutagenesis were used to introduce mutations into the 72 min region of the Escherichia coli chromosome. Twenty temperature-sensitive mutants, with linkage to markers in this region, have been examined. Each strain showed an inhibition of growth in liquid medium at 44°, and 19 of the mutants lost viability upon prolonged incubation at this temperature. A reduction in the rate of in vivo RNA and protein synthesis was observed for each mutant at 44°, relative to a control strain. Eleven of the mutants were altered in growth sensitivity or resistance to one or more of three ribosomal antibiotics. The incomplete assembly of ribosomal subunits was detected in nine strains grown at 44°. The characteristics of these mutants suggest that many of them are altered in genes for translational or transcriptional components, consistent with the clustering of these genes at this chromosomal locus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1216362Documentos Relacionados
- Isolation of Temperature-Sensitive Membrane Mutants of Escherichia coli K-12
- Temperature-sensitive autolysis-defective mutants of Escherichia coli.
- Temperature-Sensitive Osmotic Remedial Mutants of Escherichia coli
- Isolation of temperature-sensitive Abelson virus mutants by site-directed mutagenesis.
- Bacteriophage G4 DNA synthesis in temperature-sensitive dna mutants of Escherichia coli.
