Loopstructures in synthetic oligodeoxynucleotides.
AUTOR(ES)
Haasnoot, C A
RESUMO
A comparative nuclear magnetic resonance study of the hydrogen-bonded imino protons in a series of synthetic DNA fragments is presented. The fragments ATCCTA(Tn)TAGGAT are in principle capable of forming either a self-complementary hairpin loop structure (monomer form) or an interior loop structure (dimeric form). It has been shown, that for n = 1 only the dimer structure is present in aqueous solution, whereas the exclusive existence of the hairpin loop structure is indicated for n = 3, 4 & 5. Surprisingly, for n = 2 two different structures appear to be present in solution. Concentration studies show that both monomers and dimers exist side by side in this case. Hairpins as well as interior loops form extra "melting sites" in addition to the wellknown fraying phenomenon at the terminus of the double helix.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327250Documentos Relacionados
- Physicochemical properties of phosphorothioate oligodeoxynucleotides.
- Variants in clones of gene-machine-synthesized oligodeoxynucleotides.
- Antisense pro-drugs: 5'-ester oligodeoxynucleotides.
- Formation of covalent complexes between human O6-alkylguanine-DNA alkyltransferase and BCNU-treated defined length synthetic oligodeoxynucleotides.
- Facile preparation of nuclease resistant 3' modified oligodeoxynucleotides.
