Loss of Inorganic Ions from Host Cells Infected with Chlamydia psittaci
AUTOR(ES)
Chang, George T.
RESUMO
Mouse fibroblasts (L cells) infected with the 6BC strain of Chlamydia psittaci released potassium ion (K+) into the extracellular milieu in a way that depended on size of inoculum and time after infection. When the multiplicity of infection was 500 to 1,000 50% infectious units (ID50) per L cell, loss of intracellular K+ was first apparent 4 to 10 h after infection and was nearly complete at 6 to 20 h. Magnesium ion and inorganic phosphate (Pi) were also released. Similar multiplicities of ultraviolet-inactivated C. psittaci also caused release of K+. Leakage of inorganic ions probably resulted from immediate damage to the host-cell plasma membrane during ingestion of large numbers of chlamydiae. With multiplicities of 1 to 50 ID50 per L cell, ingestion of C. psittaci was not by itself enough to cause release of K+ and Pi from infected L cells. There was a delay of 36 to 72 h between infection and massive leakage of intracellular ions during which time the chlamydiae multiplied extensively. Fifty ID50 of ultraviolet-inactivated C. psittaci per L cell did not bring about significant leakage of K+, even after 72 h. The mechanism whereby these multiplicities of infection destroy the ability of host cells to retain intracellular molecules is not known. HeLa 229 cells also released K+ and Pi after infection, but these losses occurred more slowly than in comparably infected L cells, possibly because C. psittaci did not multiply as extensively in HeLa cells as it did in L cells. The significance of the inability of chlamydiae-infected cells to regulate the flow of molecules through their plasma membranes is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=422263Documentos Relacionados
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