Loss of Photoreversibility of Damage to Deoxyribonucleic Acid Replication in Ultraviolet-Irradiated Escherichia coli B/r thy trp
AUTOR(ES)
Doudney, C. O.
RESUMO
Loss of photoreversibility (LOP) of the ultraviolet (UV) damage which prevents reinitiation of deoxyribonucleic acid (DNA) replication occurred with incubation of Escherichia coli B/r thy trp cultures after UV doses of 240, 320, and 400 ergs/mm2. LOP occurred at the time of reinitiation of DNA replication in the cultures (i.e., after postirradiation lag periods of 45 min or more). Neither the absence of thymine nor the absence of tryptophan prevented LOP of the damage to DNA replication, suggesting that neither DNA replication nor protein synthesis is necessary for the process. These findings suggest that attempted initiation of DNA replication results in transformation of pyrimidine damage into permanent damage to chromosome structure at the reinitiation site.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=245775Documentos Relacionados
- Relation Between Survival and Deoxyribonucleic Acid Replication in Ultraviolet-Irradiated Resistant and Sensitive Strains of Escherichia coli B/r
- Death Through Respiratory Failure of a Fraction of Ultraviolet-Irradiated Escherichia coli B/r Cells
- Role of Pyridine Nucleotides in the Control of Respiration in Ultraviolet-Irradiated Escherichia coli B/r Cells
- Evidence Relating Cessation of Respiration, Cell Envelope Changes, and Death in Ultraviolet-Irradiated Escherichia coli B/r Cells
- Excision repair of ultraviolet-irradiated deoxyribonucleic acid in plasmolyzed cells of Escherichia coli.
