Macromolecular crowding accelerates the cohesion of DNA fragments with complementary termini.
AUTOR(ES)
Zimmerman, S B
RESUMO
Macromolecular crowding increases the rate of nonenzymatic cohesion of the complementary ends of lambda DNA. Both lambda DNA and DNA fragments bearing the cohesive ends of lambda DNA are similarly affected. High concentrations of plasma albumin or Ficoll 70 increase the rate of cohesion by ca. 100-fold whereas high concentrations of polyethylene glycol 8000 cause greater than 2000-fold stimulation in this rate. These results have implications for the mechanism of polymer-stimulated enzymatic ligation of DNA or RNA. In addition, these crowding effects may help to explain the rapid cohesion of lambda DNA observed in vivo. An improved procedure for the recovery of DNA fragments separated by agarose gel electrophoresis is also described.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=341152Documentos Relacionados
- Mapping of mycoplasma virus DNA replication origins and termini.
- Structure of the rat cytomegalovirus genome termini.
- The infectivity of adenovirus genomes lacking DNA sequences from their left-hand termini.
- Macromolecular crowding increases binding of DNA polymerase to DNA: an adaptive effect.
- Ribosome binding by tRNAs with fluorescent labeled 3' termini.