Macrorestriction Fragment Profiles Reveal Genetic Variation of Cowdria ruminantium Isolates

AUTOR(ES)

de Villiers, E. P.

FONTE

American Society for Microbiology

RESUMO

Macrorestriction profile analysis by pulsed-field gel electrophoresis (PFGE) was used to distinguish between seven isolates of Cowdria ruminantium from geographically different areas. Characteristic profiles were generated for each isolate by using the restriction endonucleases KspI, SalI, and SmaI with chromosomal sizes ranging between 1,546 and 1,692 kb. Statistical analysis of the macrorestriction profiles indicated that all the isolates were distinct from each other; these data contribute to a better understanding of the epidemiology of this pathogen and may be exploited for the identification of genotype-specific DNA probes.

Documentos Relacionados

• Size variation of the major immunodominant protein of Cowdria ruminantium.
• Sequence heterogeneity of the major antigenic protein 1 genes from Cowdria ruminantium isolates from different geographical areas.
• Amplified-Fragment Length Polymorphism Analysis versus Macro-Restriction Fragment Analysis for Molecular Typing of Streptococcus pneumoniae Isolates
• Development of an in vitro cloning method for Cowdria ruminantium.
• Identification of an immunodominant antigenically conserved 32-kilodalton protein from Cowdria ruminantium.