Male sterility and enhanced radiation sensitivity in TLS–/– mice

AUTOR(ES)

Kuroda, Masahiko

FONTE

Oxford University Press

RESUMO

TLS (also known as FUS) is an RNA-binding protein that contributes the N-terminal half of fusion oncoproteins implicated in the development of human liposarcomas and leukemias. Here we report that male mice homozygous for an induced mutation in TLS are sterile with a marked increase in the number of unpaired and mispaired chromosomal axes in pre-meiotic spermatocytes. Nuclear extracts from TLS–/– testes lack an activity capable of promoting pairing between homologous DNA sequences in vitro, and TLS–/– mice and embryonic fibroblasts exhibit increased sensitivity to ionizing irradiation. These results are consistent with a role for TLS in homologous DNA pairing and recombination.

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