Map locations of five transcripts homologous to TR-DNA in tobacco and sunflower crown gall tumors.
AUTOR(ES)
Winter, J A
RESUMO
Polyadenylated RNA from two octopine type tumor lines (E1, PSCG-15955) was analyzed by RNA blot hybridization and shown to contain five major transcripts homologous to TR DNA. In tobacco E1 tissue, the molecular weights of the TR homologous RNAs are 1.65 kb, 1.55 kb, 1.45 kb, 1.05 kb, and 0.78 kb. The 5' - and 3'-termini of each of the five E1 tumor transcripts were determined by S1 nuclease hybrid protection mapping. The polarity of transcription for the 0.78, 1.05 and 1.65 kb RNAs is from left to right, while the 1.55 and 1.45 kb RNAs are transcribed from right to left. Although we see the same size transcripts in sunflower PSCG-15955 tissue (with the exception of a 0.89 kb RNA in place of the 1.55 kb transcript of E1) there is an apparent difference in the relative abundance of the various RNAs between the two tumor lines.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=318670Documentos Relacionados
- Genetic identification of functions of TR-DNA transcripts in octopine crown galls
- Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors.
- Functional domains of a T-DNA promoter active in crown gall tumors.
- Methylation of the T-DNA in Agrobacterium tumefaciens and in several crown gall tumors.
- Genetic analysis of nonpathogenic Agrobacterium tumefaciens mutants arising in crown gall tumors.
