Mapping of a Temperate Bacteriophage Active on Bacillus subtilis
AUTOR(ES)
Rutberg, Lars
RESUMO
Bacteriophage φ105 is a temperate bacteriophage for Bacillus subtilis 168. Temperature-sensitive and plaque mutants of φ105 were isolated. The results of two- and three-factor crosses with these mutants suggest the vegetative map of φ105 to be circular. The location of prophage φ105 between bacterial markers phe-1 and ilvA1 was shown by means of PBS1 transduction. Five markers in the prophage were linearly ordered with respect to the bacterial markers. Linkage between bacterial and prophage markers was demonstrated in transformation experiments with deoxyribonucleic acid extracted from lysogenic bacteria. The data demonstrate that prophage φ105 is linearly inserted into the bacterial chromosome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=375727Documentos Relacionados
- Behavior of a temperate bacteriophage in differentiating cells of Bacillus subtilis.
- New temperate bacteriophage for Bacillus subtilis, rho 11.
- Genetic Mapping of a Defective Bacteriophage on the Chromosome of Bacillus subtilis 168
- Restriction endonuclease mapping of bacteriophage phi105 and closely related temperate Bacillus subtilis bacteriophages rho10 and rho14.
- Characterization of Infectious Deoxyribonucleic Acid from Temperate Bacillus subtilis Bacteriophage φ 105