Mapping of chromosomal IS5 elements that mediate type II F-prime plasmid excision in Escherichia coli K-12.

AUTOR(ES)

Timmons, M S

RESUMO

Three IS5 elements were mapped in overlapping chromosomal segments on a series of F-prime plasmids by restriction analysis and hybridization. IS5A was located clockwise of proA near 6 min, IS5B was located clockwise of purE near 12 min, and IS5C was tentatively located near 14 min on the Escherichia coli K-12 map. The physical structures of nine type II F-prime plasmids that contain chromosomal DNA from this region indicated that these plasmids were excised from the chromosome by recombination between pairs of IS5 elements.

Documentos Relacionados

• Escherichia coli K-12 F-prime factors, old and new.
• Further mapping of IS2 and IS3 in the lac-purE region of the Escherichia coli K-12 genome: structure of the F-prime ORF203.
• Characterization by Electron Microscopy of Fused F-Prime Factors in Escherichia coli
• Transfer-Defective Mutants of Sex Factors in ESCHERICHIA COLI. II. Deletion Mutants of an F-Prime and Deletion Mapping of Cistrons Involved in Genetic Transfer
• F-Prime Factors of SALMONELLA TYPHIMURIUM and an Inversion between S. TYPHIMURIUM and ESCHERICHIA COLI