Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites.
AUTOR(ES)
Inselburg, J
RESUMO
Colicin plasmids E2 and E3 (Col E2 and Col E3) deoxyribonucleic acid (DNA) has been shown to contain, respectively, two and three EcoR1 restriction endonuclease-sensitive sites. This was determined by measuring the DNA fragments generated after EcoR1 endonuclease treatment by agarose gel electrophoresis and electron microscopy. The structure of heteroduplex Col E2-col E3 DNA molecules formed from EcoR1-generated fragments permitted a localization of the EcoR1-sensitive sites on the plasmid chromosomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=285653Documentos Relacionados
- Discontinuous replication of colicin E1 plasmid deoxyribonucleic acid.
- Overproduction of the EcoR V endonuclease and methylase.
- Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.
- Incompatibility Exhibited by Colicin Plasmids E1, E2, and E3 in Escherichia coli
- Non-specific binding of restriction endonuclease EcoR1 to DNA.
