Mapping of the genes coding for the two major vaccinia virus core polypeptides.

AUTOR(ES)

Wittek, R

RESUMO

We have mapped the genes coding for two major structural polypeptides of the vaccinia virus core by hybrid selection and transcriptional mapping. First, RNA was selected by hybridization to restriction fragments of the vaccinia virus genome, translated in vitro and the products were immunoprecipitated with antibodies against the two polypeptides. This approach allowed us to map the genes to the left hand end of the largest Hind III restriction fragment of 50 kilobase pairs. Second, transcriptional mapping of this region of the genome revealed the presence of the two expected RNAs. Both RNAs are transcribed from the leftward reading strand and the 5'-ends of the genes are separated by about 7.5 kilobase pairs of DNA. Thus, two genes encoding structural polypeptides with a similar location in the vaccinia virus particle are clustered at approximately 105 kilobase pairs from the left hand end of the 180 kilobase pair vaccinia virus genome.

Documentos Relacionados

• Segment 8 of the influenza virus genome is unique in coding for two polypeptides.
• Physical mapping of herpes simplex virus-induced polypeptides.
• Mapping of a gene coding for a major late structural polypeptide on the vaccinia virus genome.
• Mapping of the structural gene of pseudorabies virus glycoprotein A and identification of two non-glycosylated precursor polypeptides.
• Identification of the major membrane and core proteins of vaccinia virus by two-dimensional electrophoresis.