Mapping of transcription sites of simian virus 40-specific late 16S and 19S mRNA by electron microscopy.
AUTOR(ES)
May, E
RESUMO
Simian virus 40 (SV40) late 19S and 16S mRNAs were annealed to complementary regions of partially melted viral double-stranded SV40(LHpa II) DNA or SV40(LBam HI) DNA. The RNA-DNA hybrid regions within the DNA molecules were visualized as loops [with SV40(LBam HI) DNA] in the electron microscope. The data confirm the previous localizations of the 3' and 5' ends of 16S SV40 mRNA and of the 3' end of late 19S SV40 mRNA. The 5' end of the major stable SV40 late 19S mRNA has been positioned at 0.755 map unit. Thus, the sequences of viral DNA from 0.655 to 0.755 map unit, including the replication origin, are not converted into major stable species of late viral mRNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392316Documentos Relacionados
- Transcription pattern of in vivo-labeled late simian virus 40 RNA: evidence that 16S and 19S mRNA's are derived from distinct precursor RNA populations.
- Mutational alterations within the simian virus 40 leader segment generate altered 16S and 19S mRNA's.
- Capping structures of simian virus 40 19S and 16S mRNAs.
- Sequence heterogeneity at the 5' termini of late simian virus 40 19S and 16S mRNAs.
- Translational control of synthesis of simian virus 40 late proteins from polycistronic 19S late mRNA.