Mating-Type Mutations in SCHIZOSACCHAROMYCES POMBE: Isolation of Mutants and Analysis of Strains with an h- or h+ Phenotype

AUTOR(ES)

Meade, James H.

RESUMO

Mutants defective in various steps of the sexual cycle have been isolated from homothallic strains of Schizosaccharomyces pombe by Bresch, Müller and Egel (1968). These mutants include heterothallic h+ and h- strains. We have isolated additional h+ and h- mutants from homothallic strains. Those mutants which are due to mutations in the mating-type region were analyzed in detail. Our results show that the mating-type gene mat2 not only has a function in copulation and meiosis, but that it also regulates the formation of the map1 gene product (map1 is a mating-type auxiliary gene). Some of the h - mutants have lost only one of the three functions while others are defective in at least two, and perhaps all three, functions. Further, we show that the mat1- allele of h90 strains can mutate to mat1+ but that mutations in mat2 appear to affect the mutational behavior of mat1. Finally, we describe a new inactive mating-type allele, mat2*, which is different from mat20 in that it can mutate to mat2+.

Documentos Relacionados

• Molecular and Cellular Dissection of Mating-Type Switching Steps in Schizosaccharomyces pombe
• The Mutator Gene Swi8 Effects Specific Mutations in the Mating-Type Region of Schizosaccharomyces Pombe
• Two Different h- Mating Types in SCHIZOSACCHAROMYCES POMBE
• A Suppressor of Mating-Type Locus Mutations in SACCHAROMYCES CEREVISIAE: Evidence for and Identification of Cryptic Mating-Type Loci
• Three Additional Linkage Groups That Repress Transcription and Meiotic Recombination in the Mating-Type Region of Schizosaccharomyces Pombe