Measurement of the cross linking compound, pyridinoline, in urine as an index of collagen degradation in joint disease.
AUTOR(ES)
Robins, S P
RESUMO
An enzyme linked immunoassay (ELISA) for the collagen cross link, pyridinoline, has been developed using affinity purified antibodies, with a sensitivity down to about 0.1 ng of cross link. Measurements of urinary pyridinoline were made in patients with rheumatoid arthritis (RA), osteoarthritis (OA), and a control group showing no signs of joint disease. Expressed relative to creatinine values, pyridinoline was significantly increased in both RA and OA groups compared with controls: these differences were much larger than could be attributed to any age related effects or to changes in urinary creatinine concentrations. These findings were confirmed by analysis of a series of 24 h urine collections which showed that the total pyridinoline excretions were significantly higher in both RA and OA groups than in the controls. As pyridinoline is much more prevalent in cartilage than in bone collagen, measurement of this compound in urine may provide an index for monitoring the increased joint destruction that occurs in arthritic disease.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1002034Documentos Relacionados
- Urinary excretion of hydroxylysine and its glycosides as an index of collagen degradation.
- 3-Methylhistidine excretion as an index of myofibrillar protein catabolism in neuromuscular disease.
- Accelerated collagen cross-linking in the management of advanced Acanthamoeba keratitis
- Mice lacking alpha 1 (IX) collagen develop noninflammatory degenerative joint disease.
- Radioimmunoassay for the carboxy-terminal cross-linking domain of type IV (basement membrane) procollagen in body fluids. Characterization and application to collagen type IV metabolism in fibrotic liver disease.
