Measurement of the proton motive force in Rhizobium meliloti with the Escherichia coli lacY gene product.
AUTOR(ES)
Gober, J W
RESUMO
An Escherichia coli lac operon constitutive for lacY was subcloned into the EcoRI site of a wide-host-range plasmid of the Q incompatibility group, and the resulting recombinant plasmid was introduced into Tn5-generated Lac- mutants of Rhizobium meliloti. The R. meliloti transconjugants accumulated lactose about 1,000-fold, equivalent to a proton motive force of -170 to -180 mV, not significantly different from the values calculated from the distributions of weak acids and lipophilic cations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=214343Documentos Relacionados
- Isolation, genetic analysis, and characterization of Escherichia coli mutants with defects in the lacY gene.
- Proton-motive-force-dependent step in the pathway to lysis of Escherichia coli induced by bacteriophage phi X174 gene E product.
- Mutations in the lacY gene of Escherichia coli define functional organization of lactose permease.
- lacY mutant of Escherichia coli with altered physiology of lactose induction.
- Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens
