Measurement of urinary hippuric and m-methylhippuric acids by gas chromatography
AUTOR(ES)
Buchet, J. P.
RESUMO
Buchet, J. P., and Lauwerys, R. R. (1973).British Journal of Industrial Medicine,30, 125-128. Measurement of urinary hippuric and m-methylhippuric acids by gas chromatography. A gas chromatographic method for the determination of hippuric and m-methylhippuric acids in urine is described. A known amount of heptadecanoic acid (internal standard) is added to the urine before its extraction with ethyl acetate. After evaporation of the solvent, the acids are methylated with diazomethane. The residue is taken up in methanol and injected into the gas chromatograph. The ratio of the height of the hippuric or m-methylhippuric acid peak to the height of the heptadecanoic acid peak is calculated and by reference to a calibration curve prepared in the same conditions the urine concentration of the two acids is determined.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1009493Documentos Relacionados
- Quantitative determination of hippuric and m-methylhippuric acids in urine by high-speed liquid chromatography.
- Thin-layer chromatography of hippuric and m-methylhippuric acid in urine after mixed exposure to toluene and xylene.
- Measurement by gas chromatography of urinary hippuric acid and methylhippuric acid as indices of toluene and xylene exposure.
- Absorption of m-xylene vapours through the respiratory tract and excretion of m-methylhippuric acid in urine.
- Urinary excretion of hippuric acid and m- or p-methylhippuric acid in the urine of persons exposed to vapours of toluene and m- or p-xylene as a test of exposure