Mechanism of biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme.
AUTOR(ES)
Nesbakken, T
RESUMO
The biosynthesis of 2-oxo-3-methylvalerate in Chlorobium vibrioforme was investigated by 13C nuclear magnetic resonance spectroscopy of the oxoacid formed from 13C-labeled acetate by washed suspensions. The threonine pathway could be excluded, and the results are in accord with a mechanism for the formation of 2-oxobutyrate from acetyl coenzyme A and pyruvate via citramalate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=211284Documentos Relacionados
- Transformation of glutamate to delta-aminolevulinic acid by soluble extracts of Chlorobium vibrioforme.
- Specific inhibition of antenna bacteriochlorophyll synthesis in Chlorobium vibrioforme by anesthetic gases.
- Malate dehydrogenase from the mesophile Chlorobium vibrioforme and from the mild thermophile Chlorobium tepidum: molecular cloning, construction of a hybrid, and expression in Escherichia coli.
- Cloning and expression of a structural gene from Chlorobium vibrioforme that complements the hemA mutation in Escherichia coli.
- Malate dehydrogenase from Chlorobium vibrioforme, Chlorobium tepidum, and Heliobacterium gestii: purification, characterization, and investigation of dinucleotide binding by dehydrogenases by use of empirical methods of protein sequence analysis.